Paper Details:
Nat Methods. 2007 Sep;4(9):717-9. Epub 2007 Aug 12.
Tomographic phase microscopy.
Choi W, Fang-Yen C, Badizadegan K, Oh S, Lue N, Dasari RR, Feld MS.
G.R. Harrison Spectroscopy Laboratory, 77 Massachusetts Avenue 6-014, Massachusetts Institute of Technology, Cambridge, Massachusetts 02139, USA.
We report a technique for quantitative three-dimensional (3D) mapping of refractive index in live cells and tissues using a phase-shifting laser interferometric microscope with variable illumination angle. We demonstrate tomographic imaging of cells and multicellular organisms, and time-dependent changes in cell structure. Our results will permit quantitative characterization of specimen-induced aberrations in high-resolution microscopy and have multiple applications in tissue light scattering.
Discussion leader: Shan
Time: Oct 11 10.30am
Venue: Div pantry area
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