Microscopy at 29 nm optical resolution

Dear Bioimaging People
Hello

Here is an interesting paper to discuss that I believe may generate some ideas. It is about Stimulated Emission Depletion (STED) fluorescence microscopy, invented by Stefen Hell's group in Germany, and has apparently removed the barriers of classical diffraction limit in optical resolution. In this paper they are claiming a resolution of 29 nm ( who needs near-field microscopy like NSOM or other scanning probe techniques now!). Anyway, let's talk about it on 25th.

Cheers

Shakil

Citation: "STED microscopy with continuous wave beams", Katrin I. Willig, Benjamin Harke, Rebecca Medda and Stefan W. Hell, Nature Methods 21 October, 2007.
Published online at http://www.nature.com/naturemethods

http://www.nature.com.libproxy1.nus.edu.sg/nmeth/journal/vaop/ncurrent/pdf/nmeth1108.pdf